Find sequences that match your query, which can be a protein/nucleotide string.
- Enter or copy and paste a protein/nucleotide string. See Motif Patterns.
- Select the appropriate Sequence Type based on your query.
- Check the Include NCBI Sequences box to include those sequences with NCBI (National Center for Biotechnology Information) database entries.
- Under Advanced Biosequence Search, you may change the default Query Coverage % and E-Value selections as well as check the Combine Motif Results option to return a single, unified set of results across all variants.
- Click the Search Sequences button.
You will then arrive back on the Home page where you may View Results and status for individual searches.
Important: Sequence results expire 30 days after the date they are first generated.
Motif Patterns
Amino Acid Codes
Creates a positive scoring mismatch for amino acids (e.g., A sequence query with a B will retain the B, and the B will score positively against D or N in the hit subject sequence).
Degenerate Code |
Logical Meaning |
X |
Any amino acid |
B |
D or N |
Z |
E or Q |
J |
I or L |
Nucleotide Codes
Generates multiple queries for nucleotides where the degenerate code is replaced by the nucleotides it represents.
Degenerate Code |
Logical Meaning |
N |
A or C or G or T |
R |
A or G |
Y |
C or T |
M |
A or C |
K |
G or T |
S |
C or G |
W |
A or T |
H |
A or C or T |
B |
C or G or T |
V |
A or C or G |
D |
A or G or T |
Regex Syntax
Degenerate Code |
Logical Meaning |
. |
Translated to X for protein Enumerates to A, G, C, and T for nucleotide (generates four search terms) |
{m,n} |
At least m and maximum n residues length |
{n} |
Exactly n length |
[XYZ] |
Any nucleotide or amino acid listed within the square brackets |
^XYZ$ |
Search for the exact sequence XYZ |