Find sequences that match your query, which can be a protein/nucleotide string or a .txt/.fasta file.
- Enter or copy and paste a protein/nucleotide string, or upload a sequence file:
- Single sequence: .txt file
-
Multiple sequences: .fasta file
Note: The maximum number of sequences is 100.
- Select the appropriate Sequence Type based on your query.
- Select the relevant Search Within option. Check the Include NCBI Sequences box to include those sequences with NCBI (National Center for Biotechnology Information) database entries..
- Click the Search Sequences button.
Click Advanced Sequence Search to find sequences using parameters such as sequence identity percentage, gap costs, and BLAST algorithm.
You will then arrive back on the Home page where you may View Results and status for individual searches.
Important: Sequence results expire 30 days after the date they are first generated.
Advanced Sequence Search
Advanced Sequence Search has default values based on the selected Sequence Type and Search Within options.
|
Sequence Type / Search Within |
Options (Default Value Shown) |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
Adjust Parameters for Short Sequences |
Sequence Identity % |
Match with Gaps |
Gap Costs |
Query Coverage % |
Word Size |
Reward for Match, Penalty for Mismatch |
Scoring Matrix |
BLAST Algorithm |
E-Value |
Exclude Low-Complexity Regions |
|
|
Nucleotide / Nucleotides |
Available |
80 |
No |
Existence 5, Extension 2 |
90 |
11 |
2, -3 |
— |
BLASTn |
10 |
No |
|
Nucleotide / Proteins |
N/A |
— |
No |
Existence 11, Extension 1 |
90 |
6 |
— |
BLOSUM62 |
BLASTx-fast |
10 |
— |
|
Protein / Nucleotides |
N/A |
— |
No |
Existence 11, Extension 1 |
90 |
6 |
— |
BLOSUM62 |
TBLASTn-fast |
10 |
— |
|
Protein / Proteins |
Available |
— |
No |
Existence 11, Extension 1 |
90 |
3 |
— |
BLOSUM62 |
BLASTp |
10 |
No |